Some Factors Which Affect Amino Acid Uptake by Saccharomyces carlsbergensis

[Some factors which affect the enzymatic digestion of ribonucleic acid].

The results of Klee and Richards (1) are in disagreement with the conclusions of Anfmsen et al. (2-4) that concentrated urea has no effect on the activity of bovine pancreatic ribonuclease. The possibility exists that differences in enzymatic activity might be also caused by alteration of the substrate rather than of the enzyme upon treatment with these agents. In examining the effect of condit...

Some chemical factors which influence the amino acid requirements of the lactic acid bacteria.

In developing microbiological methods for the determination of amino acids, vitamins, and other substances of nutritional importance, it is desirable first of all to have as complete and detailed information concerning the nutritional requirements of the organisms as it is possible to obtain. During the last few years, as microbiological procedures for determining amino acids have been worked o...

Subcellular fractionation of particles containing acid hydrolases from Saccharomyces carlsbergensis.

It has been shown that, under suitable conditions, the lysis of osmotically stabilized sphaeroplasts of yeast leads to the release of cell-vacuoles, which can then be purified on iso-osmotic gradients (Matile & Wiemken, 1967). On the basis of these fractionations these authors claim that the single large cell-vacuole is the only lysosome of the yeast cell. However, it has been shown that, by us...

Factors which affect cerebral uptake and retention of 13NH3.

Glucose-induced Release of Amino Acids from Saccharomyces carlsbergensis by Action on the Cytoplasmic Membrane.

When washed yeast cells grown under appropriate conditions were suspended in glucose solution there was a sudden release of alpha-amino nitrogen into the medium. This released material was of low molecular weight, and its composition was closely similar to that of the intracellular free amino acid pool. During the leakage of amino acids, the yeast did not efficiently absorb labeled amino acids ...